Our hematology services include a full range of pathology expertise and advanced laboratory diagnostics. Pediatric and adult patients with benign and malignant hematolymphoid disorders can benefit from our subspecialty services that provide accurate and timely diagnoses, assist with prognostic stratification, and guide therapy selection and monitoring.
As a leading provider of state-of-the-art laboratory diagnostics required in modern hematology practice, our menu of services is complemented by a solid foundation of expert consultation for bone marrow biopsies and lymphoid tissues.
We are able to provide data necessary to answer today's complex hematology questions through our robust offerings in flow cytometry, molecular hematology, immunohistochemistry and cytogenetics, as well as interpretation of that data by highly trained hematopathologists who add context to the results.
From performing a single test to providing full-service consultations, we offer a spectrum of services tailored to the needs of each client.
Composed of plasma (55%) and blood cells (45%)
Responsible for transport of oxygen, carbon dioxide, nutrients, cell waste, antibodies, and immune cells
Completes circulatory loop in 20 seconds
Adults have 6 liters of blood containing 35 trillion cells
Organ of T-cell production found anterosuperior to heart
Grows from 20 to 35 grams into teens, and progressively disappears ("involutes") in adulthood
Responsible for producing T-cells for cellular immunity
Multifunctional organ that houses hematopoiesis in utero
Found in upper right abdominal quadrant
Responsible for blood cell production in utero and supplemental blood filtration
An organized collection of immune cells
Responsible for immunity
Adults have 800 lymph nodes and their size is quite variable
4. Lymph Node:
Two large lymph node-like organs in oral cavity pillars
Each measure on average 2.5 - 3 cm in dimension
Responsible for immune surveillance
Blood filter found in upper left abdominal quadrant
12 cm in largest diameter and 150-200 grams
Contains red blood cell "obstacle course" and many lymph node-like immune cell collections
Responsible for clearing old red blood cells and immunity
Composed of hematopoietic cells and fat
Produces 2.5 million cells/second
Found in all bones at birth, but recedes to axial bones (pelvis, vertebra, and ribs) with age
Responsible for producing blood and immune cells, and maintaining the genome
Adults have 2 liters of bone marrow containing 2 trillion cells
Humans have 22,000 stem cells at birth
7. Bone Marrow:
5 tests in 1 lab
The appearance of cells and their relationship to one another under the microscope is the cornerstone of pathology. When abnormal cell proliferations arise, identification of the cell of origin is vital for proper disease classification and management. Cells may have a wide variety of morphologies, and their sizes, shapes, colors, nuclear and cytoplasmic features, and the company they keep often disclose their origin.
Utilizing many different histological stains, including routine stains (H&E and Wright-Giemsa), special stains (iron, trichrome, Congo Red, etc.), and immunohistochemical stains (AE1/AE3, SOX11, CD45RB, etc.), cell features can be accentuated to improve their characterization. Microscopy allows for the visual evaluation of thousands of cells, and at GoPath, our experienced eyes and extensive stain menu (>120 stains) translate into timely, accurate diagnoses.
Cells express a multitude of antigens on their surface, in their cytoplasm, and in their nuclei. What cells express and don’t express are important cell characteristics, and rapid evaluation of each cell’s antigen profile allows for population assessment and the identification of aberrancy. Flow cytometry is a powerful approach to characterize disease and steer treatment, and its utility has had no greater impact than in hematopathology.
Flow cytometry utilizes a rainbow spectrum of antigen-specific fluorescent dyes to paint cells. When these painted cells are marched single file through an interrogation chamber and illuminated by three lasers, any attached dyes are excited and fluoresce, and 12 detectors collect parameters on each cell. A computer tabulates the data, and software permits for manipulation and interpretation. This extraordinary methodology can evaluate data from more than 500,000 cells in minutes.
At GoPath®, we have state-of-the-art Navios EX cytometers, DURAClone IM Count and ClearLLab 10C dry down tube systems, wet lab reflex tube capability, and Kaluza software that we have internally programmed and standardized for optimal interpretation. Our department has more than 100,000 flow cases of experience, and we offer global or TC/PC flow options.
FISH, or fluorescence in situ hybridization, is a more sensitive examination of DNA, utilizing probes to identify genetic abnormalities. A FISH probe is a fluorescent-labeled complementary DNA fragment that adheres, or hybridizes, to a specific DNA sequence in the patient’s cells. The fluorescence highlights the target sequence and permits microscopic detection of abnormal chromosome numbers, genetic rearrangements, genetic loss, or mutated genetic sequences. FISH's sensitivity and rapid results make it a powerful tool in the diagnosis of hematopoietic diseases; however, like the sport of fishing, one must first know what they're looking for in order to select the correct probe. GoPath offers a wide variety of individual probes and panels to complement the disease workup.
Chromosome analysis, or karyotyping, involves analyzing the number and structure of chromosomes to detect abnormalities. A live cell sample is collected and cultured to promote cell division. The cell cycle is arrested in metaphase, and the isolated chromosomes are placed on a slide and stained. Each chromosome has a unique fingerprint, and they are mapped and examined for changes in number and arrangement. Karyotyping looks for gross structural abnormalities in the DNA and is essential for the proper diagnosis of acute leukemias and myelodysplastic syndromes.
Molecular pathology has a fundamental role in hematopathology and has driven many of the most significant trends of late. More sensitive testing, more data at diagnosis, expansion of disease classification systems, recognition of more molecular-defined diseases, development of precision therapeutics, greater molecular prognostic focus, and more sensitive monitoring methods can all be attributed to advances in molecular pathology.
Molecular testing is the most sensitive inspection of DNA as it focuses on the DNA sequence and the identification of mutations. The days of "the diagnosis is the endpoint" have passed, and molecular testing plays an important role in the classification and management of hematologic disorders. GoPath offers single and multiple gene tests, as well as targeted next-generation sequencing panels to complete your hematopathology workup.
The leading choice of pathologists, clinicians and patients who seek an expert opinion in hematopathology
GoPath Diagnostics offers physicians an unsurpassed level of expertise, state-of-the-art scientific analysis and personal attention. Guided by the vision and leadership of Dr. Jim Lu and Dr. David Wilson, we offer referring physicians the speed and accuracy of results required to best assist their patients.
With our full-service consultative services, we provide a single, comprehensive hematopathology consultation report containing detailed diagnostic and prognostic information. For patients with multiple samples, a systematic comparative evaluation is used to address issues such as disease progression and response to therapy. This approach is critical in view of the complicated diagnostic algorithms necessary to apply the current WHO (2022) criteria, especially in cases of organ-based lymphomas, lymphomas in immunocompromised patients, and in many myeloid neoplasms. Telephone consultations are encouraged and a dialogue with the clinician may even be necessary to reach a comprehensive interpretation and to understand the full implications of the diagnosis.
Consultation and case review by international experts in hematopathology—difficult cases are discussed in a consensus conference. Clients benefit from the expertise of five hematopathologists without incurring additional fees.
State-of-the-art flow cytometry, immunohistochemistry and molecular laboratories.
Efficient personal attention and timely reporting.
Specimen shipping at no cost to the physician or patient. For consultation cases, all tissue block(s) and original slides are promptly returned.
Referring physicians and their patients benefit from:
Determined through comprehensive assessment of all tests performed
Cytologic and histologic evaluation of cells and tissue by microscopy
Immunophenotypic characterization of all cell populations
Analysis of number and structure of chromosomes
Identification of specific genetic abnormalities using fluorescent-labeled probes
Single or multiple gene sequencing to identify genomic aberrations
Dr. Wilson earned his medical degree and completed his residency in Clinical and Anatomic Pathology, and his fellowship in Hematopathology at the Indiana University School of Medicine. Dr. Wilson is a Chairman of the Department of Pathology at St. Vincent Hospital and a Hematology and Blood Bank instructor at Marion University College of Osteopathic Medicine. He is a member of several societies, including the College of American Pathology (CAP), the Society for Hematopathology, and the United States and Canadian Academy of Pathologists (USCAP). Dr. Wilson has been published in the American Journal of Clinical Pathology and the ACC Current Journal.
Dr. Wilson has practiced hematopathology and surgical pathology for more than 20 years in Indianapolis, where under his direction, he grew a small local hematology laboratory into a busy midwestern Hematopathology Service that has averaged more than 7,000 blood smear reviews, 2,000 bone marrows, 5,000 flow cytometry cases, 5,000 heme-related surgicals, and 6,000 coagulation consults per year.
As a frequent and active participant in annual international pathology and clinical hematology conferences, Dr. Wilson has maintained this state-of-the-art service in an increasingly complex hematopathology landscape.
To the clinicians he has served, Dr. Wilson is well known for his sense of humor, tireless work ethic, efficiency, accessibility, staunch patient advocacy, timeliness, and mastery.
Hematology Diagnostic Services
Providing our clients with world-class hematology diagnostics and laboratory medicine services is our passion. The Department of Hematopathology delivers total customer satisfaction with its quick turnaround time, friendly Client Services department, and on-time billing.
5 tests in 1 lab
More than 100 IHCs
The story of our full-service diagnostics laboratory begins with